BioSB RNA- seq data analysis course (5th edition)

Mon 7 September 2015 to Wed 9 September 2015
Leiden, Netherlands

BioSB and Leiden University Medical Centre (LUMC) are organizing a 3-day course on RNA-seq. This is an expert course for people with experience in NGS and a follow-up course on the general NGS data analysis course (coordinator Jeroen Laros, LUMC). The course will consist of seminars and hands-on command line, Galaxy and R practicals and will focus on data preprocessing, quality control, alignment, visualization and statistics for differential transcript expression. Examples will be taken from human and mouse studies. The course does not cover prokaryotic RNA profiling nor plant- and metagenomics aspects.

Course topics 

  • RNA-seq experimental approaches

  • Alignment and de novo assembly

  • Statistics for differential gene expression

  • eQTL analysis and allele specific expression

  • Variant calling and RNA editing

  • Small RNA profiling

  • Software for RNA-seq data analysis


The tentative programme can be found at:

Target audience

Participants for the RNA-seq course should preferably have participated in the general NGS course or otherwise have demonstrated hands-on experience with NGS data analysis. The course is aimed at PhD students and post-docs, but scientific programmers and data analysts with a background in biology and bioinformatics may also attend. Registrations will be handled on a first-come-first-served basis but experience and motivation will be reviewed. Seats have been reserved for participants in the FP7-funded project RD-Connect and Marie Curie European Training Network TranCYST.

BioSB Course Programme

This course is part of the Course Portfolio of BioSB, the Netherlands Bioinformatics and Systems Biology research school, which offers  training and education for in bioinformatics and systems biology.
More information about BioSB can be found at

For more information about the course you can contact Celia van Gelder at education [at]