Organised by ELIXIR Italy in collaboration with CINECA and Sapienza University of Rome, this training course will introduce the participants to the primary analysis of datasets generated through the RNA-Seq assays. The topics covered by the programme:

• High-throughput sequencing (HTS) technologies and their application to gene expression analysis (RNA-Seq)
• key steps in the data analysis pipeline (such as quality control, alignment, summarization) along with most commonly used software
• Dataset specific downstream analysis, including quantification of expression, transcriptome assembly and differential expression analysis

This course is aimed at PhD students and post-doctoral researchers who are considering or already generating next-generation sequencing datasets, but have no or limited experience in data analysis. Participants with no UNIX/Linux experience will be provided with basic understanding of the command-line operations in the first day of the course.